Arezoo Tooghi; Gholam Dashab; Ali Maghsoudi; Mohammad Rokouei
Volume 24, Issue 2 , July 2022, , Pages 127-138
Abstract
The aim of this study was to investigate the genetic diversity of different camel breeds in Sistan and Baluchestan province using microsatellite markers. For this purpose, 70 camels were randomly selected from Jamazeh and Baluchi breeds. Jugular vein blood sampling was performed in the tubes containing ...
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The aim of this study was to investigate the genetic diversity of different camel breeds in Sistan and Baluchestan province using microsatellite markers. For this purpose, 70 camels were randomly selected from Jamazeh and Baluchi breeds. Jugular vein blood sampling was performed in the tubes containing 0.5% EDTA. The extraction of DNA was performed by salt-detergent method. Five microsatellite markers were selected from the camel genome project with specific primers and banding patterns on 5% agarose gel. Genetic and population structures were analyzed using POPGENE v1.31 software. The number of alleles in five marker positions in Jamazeh and Baluchi breeds ranged from one to six alleles. The number of alleles in five markers CVRLO6, VOLP32, LCV66, WYLLO and CMS17 in Jamazeh are equal to three, two, three, four and one alleles, respectively, and in Baluchi breed with two, four, two, five and one alleles, respectively. According to the results, the range of heterozygosity observed in the studied sites in Jamazeh and Baluchi breeds was in the range from 0.29 to 0.52. The results of FST analysis showed that the two populations were more than 96% similar and the difference between them was 3%. The results of the presentresearchindicated a low rate of polymorphism in the studied sites and in order to preserve breeds and be more adaptable to environmental conditions, it needs more attention and control of inbreeding.
Vahideh Sabaghi-Darmiyan; Mehran Mehri; Farzad Bagherzadeh Kasmani; Mohammad Rokouei
Volume 23, Issue 2 , July 2021, , Pages 281-292
Abstract
The experiment was carried out to investigate the effect of tryptophan, melatonin and dimethylglycine on the growth performance and meat quality of Japanese quails fed diets contaminated with aflatoxin B1 toxin. In this experiment, 680 Japanese quail from 7 to 35 days of age were allotted to 17 ...
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The experiment was carried out to investigate the effect of tryptophan, melatonin and dimethylglycine on the growth performance and meat quality of Japanese quails fed diets contaminated with aflatoxin B1 toxin. In this experiment, 680 Japanese quail from 7 to 35 days of age were allotted to 17 treatments and 4 replicates (10 birds per replication), and trial performed using a central composite design. The performance of birds was calculated on a weekly basis and at the end of the experiment; and the amount of malondialdehyde in meat was determined. Results showed that tryptophan increase weight gain and melatonin had an increased effect on feed intake (P < 0.05). The effect of tryptophan and as well as the interaction of tryptophan and dimethylglycine reduced the amount of malondialdehyde in meat (P < 0.05). The results obtained from this research state that dietary supplementation with tryptophan and methyl group donors have good antioxidant capacity to reduce aflatoxin B1 poisoning on growth performance and malondialdehyde content of meat, and the use of these supplements in the diet of Japanese quails seems desirable.
batol asghari esfedan; Gholam Dashab; mohammadhossein banabazi; mohammad Rokouei
Volume 22, Issue 2 , June 2020, , Pages 187-198
Abstract
The aim of the present study was to investigate the codon usage pattern and their relationship with gene expression for genes with different expression between pure and crossbreed Sistani and Montbeliared breed. In this study, the results of differential gene expression analysis using RNA-Seq technology ...
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The aim of the present study was to investigate the codon usage pattern and their relationship with gene expression for genes with different expression between pure and crossbreed Sistani and Montbeliared breed. In this study, the results of differential gene expression analysis using RNA-Seq technology between pure and crossbreed Sistani and Montbeliared breed (two pure and two its crossbreed) were used. For this purpose, after determining the ORF regions for these genes, CodonW software was used to estimate codon usage pattern indices including CAI, ENC, GC and GC3s. Results showed that there was a significant correlation between total GC and GC3s (0.74). There was also a significant correlation between ENC and GC and GC3s (0.65, 0.77), indicating the role of mutation in codon formation. Based on the results of this study, the factors such as nucleotide composition (GC content), mutation, and gene expression level played important roles in codon formation in the genes studied in this study. This study is the first comparison between pure and crossbreed Sistani and Monti-billiard samples, which helps to better understand the evolutionary mechanisms of codon usage pattern formation and its association with gene expression.